FCCC    Clusters of Antibody CDR Loop Conformations     Dunbrack Lab
 
    

Search on Database

Users can search on the database by three ways: PDB, Cluster Code ,CDR and Germline

  • PDB

    Input a PDB code and/or chain ID to find the antibody CDRs. The PDB code is not case sensitive while the chain is case sensensitive. That is, 1P7K is same as 1p7k, but 1P7KL is not same as 1P7Kl. Figure 1 shows the PDB input page.

    PDB Search

    Figure 1: PDB input page

    Figure 2 shows the search resultfor 1P7K and chain L. There are 3 CDRs: L1, L2, L3, and 3 clusters: L1-11-1, L2-8-1 and L3-9-cis7-1. Cluster is formated as CDR-length-cluster number.

    Result for PDB search

    Figure 2: CDRs and clusters for 1P7K and chain L.

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  • Cluster Code

    Figuer 3 shows all clusters in 6 CDRs. Cluster with * are new CDR lengths or cis/trans conformations of existing lengths that have not yet been clustered. Reclustering of existing lengths and clustering of new lengths will occur by the Antibody Team every few months once enough new data is available from the PDB. Select a cluster to retrieve the structures with CDRs in the cluster.

    Cluster Search

    Figure 3: Cluster select page.

    Figure 4 shows a summary table for the input cluster L1-11-1 and all structures in the cluster. Click "Show Non-Redundant Chains Only" to display only the structures with unique sequences and best resolutions. Click the same button to display all structures again.

    "Export csv File" is to export the data in the table into a csv file which can be saved into the local machine.

    Result for Cluster Search

    Figure 4: The structures in cluster L1-11-1

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  • CDR

    Select a CDR to retrieve all structures containing the CDR. Figure 5 shows all CDRs and their lengths.

    CDR Search

    Figure 5: CDR select page.

    Figure 6 shows the structures and CDR clusters for CDR L1. "Show Non-Redundant Chains Only" is to show the structures with unique sequences and best resolutions. Click the same button to display all structures again.

    "Export csv File" is to export the data in the table into a csv file which can be saved into the local machine.

    Result for CDR Search

    Figure 6: Structures and clusters with CDR L1.

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  • Germline

    Select a species and germline to retrieve framework/CDRs information of the PDB structures with the species and germline.
    There are 3 ways to choose a germline:

    1. Framework

    The species and germline of the framework of PDB structures are the selected species and germline, while the species and germlines of CDRs may not same as the frameworks'.

    2. CDRs

    The species and germline of the CDRs of PDB structures are the selected species and germline, while the species and germlines of frameworks may not be same as the CDRs'.

    3. Both

    The species and germline of both framework and CDRs are the selected species and germline. So they are always same.

    Figure 7 shows the all germlines in the current databases. For each item, the first word is species, the second one is germline IG class.

    Germline Search

    Figure 7: Germline select page.

    Figure 8 shows the species, germlines and clusters of both framework and CDRs for Human species and germline IG class IGHV1. The framework and CDRs are from the same species and germlines. CDR species and germline is for both CDR1 and CDR2.

    Result for Germline Search

    Figure 8: The result table of germline search: Human + IGHV1 for both framework and CDRs.

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